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Aptamer-Mediated Energy Acceptor Sensitization Strategy Driving Reverse Dual-Signal Change for Constructing

Wei Jia1,2, Xiaohui Kang1,2, Zhenzhen Xie1,2

  • 1Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.

Analytical Chemistry
|March 4, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a novel upconversion nanoprobe using aptamer-mediated energy transfer for highly sensitive detection. The new design enhances signal contrast, overcoming limitations in traditional upconversion nanoprobes for improved sensing applications.

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Area of Science:

  • Nanotechnology and Materials Science
  • Analytical Chemistry and Sensing
  • Biomedical Engineering

Background:

  • Upconversion nanoprobes (UCNPs) offer low background interference and near-infrared excitation for sensing.
  • Current UCNPs primarily use luminescence resonance energy transfer (LRET) with single signals.
  • Limited LRET efficiency in UCNPs restricts signal contrast and sensitivity.

Purpose of the Study:

  • To develop a highly sensitive upconversion nanoprobe with enhanced signal contrast.
  • To introduce an aptamer-mediated energy acceptor sensitization strategy for reverse dual-signal changes.
  • To demonstrate the nanoprobe's efficacy in detecting trace levels of Pb2+.

Main Methods:

  • Fabrication of the nanoprobe by conjugating a Cy3 dye-labeled aptamer to UCNPs.
  • Utilizing the structural transition of aptamers into G-quadruplexes upon Pb2+ binding.
  • Activating LRET from UCNPs to Cy3 dye, leading to luminescence quenching and sensitization.

Main Results:

  • The nanoprobe exhibited a reverse dual-signal change: quenched green luminescence (540 nm) and sensitized Cy3 fluorescence (565 nm).
  • A detection limit as low as 51 pM for Pb2+ was achieved using the I540/I565 ratio.
  • The probe demonstrated high selectivity, anti-interference capability, and excellent performance in complex samples.

Conclusions:

  • The aptamer-mediated energy acceptor sensitization strategy effectively overcomes signal contrast limitations in UCNPs.
  • This versatile approach significantly enhances the sensitivity of upconversion nanoprobes.
  • The developed nanoprobe shows great promise for detecting trace contaminants like Pb2+ in environmental and agricultural samples.