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Published on: February 10, 2020
Research advances in capillary gel electrophoresis for detecting biomacromolecules
Wen-Jing Liu1, Ying Zhang2, Qiu-Yang Li1
1College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China; Institute of Materia Medica, Gansu Provincial Academy of Medical Sciences, Lanzhou 730000, China; CAS Key: Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, China.
Abstract:
Capillary gel electrophoresis (CGE) has been employed for the separation of biomacromolecules for over thirty years. Thanks to advances in gel materials and the ongoing development of electrophoresis instruments, the CGE method has now become one of the recognized gold standards for routine separation and analysis of biomacromolecules. This is due to its superior separation capabilities and the high robustness and reliability of its results. This paper reviews the research progress and applications of CGE in the separation and analysis of biomacromolecules, including proteins, nucleic acids, and polysaccharides, between 2015 and 2025, and offers a prospect on the future development of this technology.
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