Related Experiment Video
Updated: Mar 6, 2026

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
Published on: August 19, 2013
Chromaeus: a browser-based analytical platform for quantitative colorimetric measurement in digital chemistry
Hichem Moulahoum1, Faezeh Ghorbanizamani1
1Department of Biochemistry, Faculty of Science, Ege University, Bornova, Izmir, 35100, Turkiye. hichem.moulahoum@ege.edu.tr.
Chromaeus is a new, free online platform that standardizes digital colorimetry for analytical assays. It offers integrated tools for quantitative analysis, improving accuracy and traceability without specialized equipment.
Area of Science:
- Analytical Chemistry
- Digital Imaging
- Spectrophotometry
Background:
- Digital colorimetry is vital in analytical assays but suffers from inconsistent sampling and fragmented workflows.
- Lack of analytical traceability hinders reliable quantitative colorimetric measurements.
- Existing tools often require dedicated instrumentation or proprietary software, limiting accessibility.
Purpose of the Study:
- To introduce Chromaeus, a unified, browser-based platform for quantitative digital colorimetry.
- To overcome limitations of current digital colorimetry practices, including sampling, workflow fragmentation, and traceability.
- To provide an instrument-independent solution for standardized colorimetric analysis.
Main Methods:
- Development of Chromaeus, a freely accessible, browser-based platform with integrated tools.
- Benchmarking Chromaeus for technical reliability across various image formats, resolutions, and browsers.
- Validation of analytical applicability using a hydrogen peroxide assay and comparison with UV-Vis spectrophotometry.
- Evaluation of robustness using diverse imaging conditions and independent datasets.
- Comparative analysis against established tools like ImageJ, Photometrix, and Trigit.
Main Results:
- Chromaeus demonstrated high technical reliability, preserving colorimetric integrity (ΔE < 1; ICC > 0.99).
- Digital metrics from Chromaeus showed strong agreement with UV-Vis spectrophotometry in a hydrogen peroxide assay, with comparable limits of detection and linearity.
- The platform exhibited robust quantitative behavior under variable imaging conditions and aligned with reference RT-PCR trends.
- Comparative benchmarking revealed Chromaeus uniquely supports integrated quantitative workflows, unlike simple color extraction tools.
Conclusions:
- Chromaeus provides a generalizable framework for standardized, instrument-independent quantitative colorimetric analysis.
- The platform enhances analytical traceability and unifies fragmented workflows in digital colorimetry.
- Chromaeus offers a reliable and accessible solution for diverse quantitative colorimetric applications.
More Related Videos
09:51TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples
Published on: September 19, 2025
10:24Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
Published on: June 7, 2018
Related Concept Videos
UV–Vis Spectrometers
Quantitative Analysis
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
Complexometric Titration: Overview
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...