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Related Experiment Video

Updated: Mar 6, 2026

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
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Interface-Sensitive Epi-Coherent Anti-Stokes Raman Scattering Microscopy for Imaging Cell Adhesion Dynamics.

Mingmin Zhou1, Bin Dong1,2, Laura Lukov1

  • 1James Tarpo Jr. and Margaret Tarpo Department of Chemistry, Purdue University, 560 Oval Dr., West Lafayette, Indiana 47907, United States.

Analytical Chemistry
|March 5, 2026
PubMed
Summary
This summary is machine-generated.

Coherent anti-Stokes Raman scattering (CARS) microscopy offers label-free imaging of cell adhesion dynamics. This technique precisely visualizes cell-substrate interactions and lipid droplets, revealing new insights into cell behavior during mitosis and spreading.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Microscopy

Background:

  • Understanding cell adhesion dynamics is crucial for cell migration and extracellular matrix interactions.
  • Conventional microscopy methods lack chemical information or require fluorescence labeling for interfacial studies.

Purpose of the Study:

  • To develop a label-free microscopy technique for studying cell adhesion dynamics at interfaces.
  • To investigate the precision of epi-CARS in measuring cell-substrate distances and imaging lipid droplets.

Main Methods:

  • Utilized homodyne epi-coherent anti-Stokes Raman scattering (epi-CARS) microscopy focused at interfaces.
  • Employed a confocal epi-CARS system with a pinhole to reject out-of-focus reflections.
  • Applied the method to study cell-substrate adhesion dynamics in mitotic cells.

Main Results:

  • Epi-CARS demonstrated intrinsic interfacial selectivity with negative contrast at cell attachment areas, precisely measuring cell-substrate distances.
  • The technique provided high contrast imaging of cellular lipid droplets.
  • Observed adhesion site splitting during mitosis and outward development during postmitotic spreading.
  • Distinguished homogeneous adhesion at leading edges and heterogeneous patterns at retracting edges after mitosis.

Conclusions:

  • Epi-CARS microscopy is a powerful label-free tool for studying cell adhesion dynamics and lipid droplet distribution.
  • The method offers high precision in measuring nanometer-scale distances at cell-substrate interfaces.
  • Revealed dynamic changes in cell adhesion patterns during mitosis and cell spreading.