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The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Published on: October 5, 2016
VlWRKY71 mediates MeJA-induced terpene biosynthesis for aroma enhancement during grape berry ripening
Qiuyu Guo1, Minglan Chen1, Wenting Zhang1
1College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China.
Abstract:
Methyl jasmonate (MeJA) regulates climacteric fruit ripening, but its non-climacteric grape mechanisms are unclear. We treated 'Zhuosexiang' (V. vinifera L. × V. labruscana Bailey) grapes with MeJA at pre-veraison, tracking physiology, hormones, metabolites and transcripts. The results show that treated berries ripened faster, as shown by earlier color break and higher sugar content. MeJA triggered a transient spike in jasmonic acid-isoleucine (JA-Ile) at veraison, followed by a decline, while abscisic acid (ABA) increased gradually and remained at high levels. Weighted gene co-expression network analysis (WGCNA) identified a hub gene, VlWRKY71, which binds W-box motifs in the promoter of VlDXS1. VlWRKY71 expression correlated with JA-biosynthetic VlLOX1/VlOPR3, suggesting a JA feedback loop. Overall, VlWRKY71 links MeJA perception to terpene production and ripening.
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