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Published on: December 15, 2017
Recruiting orthogonal biological systems for engineering microorganisms
Kaifang Liu1, Zhilan Zhang2, Guangjie Liang3
1School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, China; Key Laboratory of Industrial Synthetic Biology of Jiangsu Province, Jiangnan University, Wuxi, China; School of Food Science and Technology, Jiangnan University, Wuxi, China.
Abstract:
Microbial synthetic biology focuses on the application of rational engineering strategies to reprogram microbial cells, thereby providing them with novel functions to meet different requirements. However, this engineering process inherently disrupts natural metabolism, leading to increased complexity and unpredictability within the metabolic system. To address these challenges, a series of orthogonal strategies has been developed and implemented in the construction of orthogonal genetic systems, metabolic pathways, energy systems, and regulatory systems. This review summarizes recent advances and applications of orthogonal strategies in microbial synthetic biology. Finally, future research directions in orthogonal microbial synthetic biology are explored, aiming to provide new insights for future studies.
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