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Related Experiment Video

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[Development and distribution of an inter-laboratory comparison test for HPV testing].

Marty Poy1, Kilian Jacquot1, Sandy Gross2

  • 1Centre national de référence Papillomavirus, CHU de Besançon, Besançon, France.

Virologie (Montrouge, France)
|March 6, 2026
PubMed
Summary

This study assessed HPV test reproducibility for cervical cancer screening in France. While HPV16/18 were consistently detected, other HPV types showed variability between labs and kits, impacting screening consistency.

Keywords:
HPV testInter-laboratory controlreproducibilityscreening

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Area of Science:

  • Medical Diagnostics
  • Virology
  • Public Health

Background:

  • Cervical cancer screening relies on Human Papillomavirus (HPV) testing.
  • Optimal and consistent screening requires high performance and inter-laboratory reproducibility of HPV tests.
  • Variability in HPV test performance could impact screening effectiveness across France.

Purpose of the Study:

  • To evaluate the inter-laboratory reproducibility of HPV tests used in French cervical cancer screening.
  • To assess the performance of different commercial HPV testing kits across multiple laboratories.
  • To identify potential variations in the detection of specific HPV genotypes.

Main Methods:

  • Development and distribution of an Inter-Laboratory Comparison (ILC) test panel to 25 volunteer laboratories.
  • The panel included samples with HPV-, HPV16+, HPV18+ cells, and HPV33, 39, 52, 56 plasmids.
  • Twenty-three laboratories performed HPV screening using various commercial kits.

Main Results:

  • All participating laboratories consistently detected HPV16 and HPV18 genotypes.
  • Detection of HPV33, 39, 52, and 56 (in plasmid form and lower quantities) was uneven across laboratories and kits.
  • Variations in detecting non-HPV16/18 genotypes were observed, dependent on the specific kit and laboratory.

Conclusions:

  • HPV tests demonstrate consistent detection for HPV16 and HPV18.
  • Inter-laboratory variability exists for detecting less common HPV genotypes, influenced by commercial kits.
  • These variations may not necessarily compromise the overall effectiveness of cervical cancer screening programs.