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Protocol for generating a 3D hydrogel-based tumor resection model in vitro using pancreatic cancer cells
Lea Miebach1, Marten Hagge2, Linus Hübner3
1ZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Street 2, 17489 Greifswald, Germany; Department of Hematology and Oncology, Greifswald University Medical Center, Sauerbruchstr., 17475 Greifswald, Germany.
STAR Protocols
|March 6, 2026
Summary
This study introduces two 3D in vitro models for pancreatic cancer resection. These models allow researchers to study cellular responses at tumor margins and analyze treatments effectively.
Area of Science:
- Oncology
- Biotechnology
- Biomedical Engineering
Background:
- Tumor resection margins are critical for patient outcomes.
- Accurate in vitro models are needed to study cellular responses at these margins.
- Current models lack standardization and high-throughput capabilities.
Purpose of the Study:
- To present a protocol for generating two distinct 3D in vitro tumor resection models.
- To enable the evaluation of cellular responses at tumor margins.
- To facilitate reproducible, high-throughput analysis of post-resection responses.
Main Methods:
- Patterning pancreatic cancer cells (Panc-01) in hydrogels using a custom 96-pin metal lid.
- Creating standardized resection cavities within the hydrogel.
- Refining the protocol to simulate narrow surgical resection margins.
Main Results:
- Successfully generated two distinct 3D in vitro tumor resection models.
- Established a reproducible method for creating standardized resection cavities.
- Developed a protocol adaptable to diverse treatment modalities.
Conclusions:
- The presented protocol offers a robust platform for studying tumor biology at resection margins.
- These models support high-throughput screening of therapeutic interventions.
- This work advances the field of in vitro cancer research and drug development.
