Related Experiment Video
Updated: Mar 8, 2026
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
Published on: December 17, 2019
Protocol to generate immunocompetent human heart-macrophage assembloids for the study of immune-cardiac interactions
Colin O'Hern1, Mia Dionise2, Sammantha Caywood3
1Institute for Quantitative Health Science and Engineering, Michigan State University, East Lansing, MI, USA; Department of Biomedical Engineering, College of Engineering, Michigan State University, East Lansing, MI, USA; College of Osteopathic Medicine, Michigan State University, East Lansing, MI, USA.
None:
Here, we present a protocol to generate fully human pluripotent stem cell (hPSC)-derived human heart-macrophage assembloids. We describe steps for differentiating hPSCs into monocytes, generating cardiac organoids, integrating CD14+ monocytes to become cardiac tissue-resident macrophages, and maturing the assembloids with metabolic cues. Furthermore, we provide a use-case example of disease modeling for the study of atrial arrhythmias. The system provides a reproducible, scalable, and efficient platform for studying immune-cardiac interactions, inflammation, and arrhythmogenesis. For complete details on the use and execution of this protocol, please refer to O'Hern et al.1.
