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Forces that shape the transcriptome: Linking cellular mechanosensing to mRNA splicing.

Pavel Simara1, Cristina Mazzotti2, Gokula Narayanan1

  • 1International Clinical Research Center (ICRC), St Anne's University Hospital, Brno, Czech Republic; Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

The Journal of Biological Chemistry
|March 9, 2026
PubMed
Summary
This summary is machine-generated.

Mechanical signaling, once thought limited to specific cells, is universal in eukaryotes. Mechanical stress impacts messenger RNA (mRNA) maturation and splicing, revealing a fundamental cellular process.

Keywords:
RNA maturationRNA-binding proteinsalternative splicingmechanical stressmechanosensing

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biophysics

Background:

  • Mechanical signaling is traditionally associated with specialized mechanocytes.
  • Emerging evidence indicates mechanical signaling is a universal eukaryotic cell feature.
  • Mechanical stress influences fundamental cellular processes like metabolism and intracellular signaling.

Purpose of the Study:

  • To review the literature on mechanical control of mRNA splicing.
  • To provide a comprehensive overview of mechanical signaling in mRNA maturation.
  • To highlight the significance of mechanical control in cellular biology.

Main Methods:

  • Literature review of studies investigating mechanical stress and mRNA processing.
  • Analysis of data linking mechanical signaling to RNA-binding protein organization.
  • Synthesis of evidence for mechanical influence on mRNA splicing.

Main Results:

  • Mechanical stress alters mRNA maturation in various cell types.
  • Changes in mRNA maturation are linked to the 3D reorganization of RNA-binding proteins.
  • Mechanical signaling plays a crucial role in regulating gene expression post-transcriptionally.

Conclusions:

  • Mechanical signaling is a fundamental cellular process affecting mRNA maturation and splicing.
  • The 3D spatial organization of RNA-binding proteins is a key mediator.
  • Understanding mechanical control of mRNA processing is vital for cellular biology.