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Updated: Mar 10, 2026
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
Published on: November 8, 2016
Engineering of induced pluripotent stem cells for the efficient development of non-alloreactive, hypoimmunogenic
Alexandros Nianias1,2, Afroditi Katsarou1,2, Henk Jan Prins1,2
1Amsterdam University Medical Centers (UMC) location Vrije Universiteit Amsterdam, Departement of Hematology, Amsterdam, Netherlands.
Background:
Induced pluripotent stem cells (iPSCs) are a promising platform to produce "off-the-shelf" chimeric antigen receptor (CAR)-engineered T cells (CAR-T) with thepotential for multiplex genetic engineering.
Methods:
Here, we employed genome editing to knock out the TRAC and B2M genes in iPSC lines, while simultaneously harnessing the edited loci to introduce a drug-inducible CAR and the human leukocyte antigen (HLA)-E single chain trimer.
Results:
The inducible CAR expression allowed the robust generation of T-cell receptor (TCR)-negative CD8ab+ CAR-T cells with demonstrable anti-tumor efficacy and lack of alloreactivity. HLA-class Inegative, HLA-Epositive CD8 CAR-T cells were protected against immune rejection; however, disruption of B2M resulted in genomic instability and affected the efficiency of T-cell development and the functionality of the generated T cells.
Discussion:
Facilitating multiplex engineering at well-characterized genomic loci and regulation of possible developmental effects will empower the use of engineered iPSC as a viable method to efficiently produce "off-the-shelf" CART cells.
