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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

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Published on: October 31, 2015

Compact Spectral Encoding Microscopy by Terrace Grating Optics.

Ori Refael Cohen¹, Reut Orange Kedem¹, Leonid Leites¹

¹Russel Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa 3200003, Israel.

|March 9, 2026

Summary

This summary is machine-generated.

Researchers developed Terrace gratings, 3D-printable optics for microscopes. These elements enable continuous spectral encoding, offering a simpler, more versatile alternative for advanced imaging applications.

Keywords:

3D imaging Diffraction Gratings Diffractive optical element Optical Microscopy Point spread function Engineering

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Area of Science:

Optics and Photonics
Microscopy
Biomedical Imaging

Background:

Multispectral imaging is crucial in microscopy but often requires complex optics or limits spectral range.
Existing solutions can be bulky, expensive, or lack continuous spectral coverage.

Purpose of the Study:

To introduce the Terrace grating optics family as a simple, add-on component for continuous spectral encoding in standard microscopes.
To demonstrate the versatility and efficiency of Terrace gratings for various imaging tasks.

Main Methods:

Development of flat, 3D-printable Terrace grating elements with variants for single-order and dual-order operation.
Utilizing a Fourier-optics model to establish the relationship between grating parameters and wavelength displacement.
Experimental validation using snapshot decoding of mRNA barcodes and multicolor imaging of C. elegans.

Main Results:

Demonstrated continuous spectral encoding with linear control over wavelength displacement via grating parameters.
Successfully applied Terrace gratings for rapid, single-exposure multicolor imaging of biological samples.
Showcased simultaneous depth and color readout using spectral and depth encoding masks.

Conclusions:

Terrace gratings provide a practical, plug-and-play solution for continuous multispectral imaging in conventional microscopes.
These gratings offer an efficient alternative to traditional prisms and blazed gratings.
The technology enables advanced imaging capabilities, including multicolor and depth-resolved analysis.