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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Mar 10, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Recent Advances in Single-Molecule Localization Based Super-Resolution Imaging.

Yen Leng Pak¹, Yingying Jing¹, Wenxiu Wu¹

¹College of Biological and Chemical Engineering, Qilu Institute of Technology, Jinan, China.

Small (Weinheim an Der Bergstrasse, Germany)

|March 9, 2026

Summary

This summary is machine-generated.

Single-molecule localization microscopy (SMLM) offers advanced imaging of biological structures beyond diffraction limits. This review covers SMLM principles, recent correlative methods, probe improvements, and applications in cell biology and pathology.

Keywords:

biological applications correlative methods single‐molecule localization microscopy super‐resolution imaging switching probes

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Published on: December 1, 2016

Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Single-molecule localization microscopy (SMLM) has revolutionized biological imaging over the past 20 years.
It enables visualization of structures beyond the diffraction limit, offering higher resolution and clarity.

Purpose of the Study:

To review the fundamental principles and implementation of SMLM.
To highlight recent advancements and correlative methods in SMLM over the last five years.
To discuss improvements in fluorescent probes and their demands.

Main Methods:

Review of SMLM principles and localization techniques.
Analysis of correlative SMLM methods with other imaging approaches.
Discussion of fluorescent probe development and requirements.

Main Results:

SMLM provides brighter and crisper single-molecule level imaging.
Recent SMLM developments focus on enhanced resolution, penetration depth, speed, and throughput.
Significant achievements in visualizing intracellular structures and processes across various biological systems.

Conclusions:

SMLM is a powerful tool for clarifying complex intracellular structures and dynamic processes.
Continued advancements in probes and techniques promise further breakthroughs in biological imaging.
Future trends focus on overcoming current challenges in SMLM implementation and application.