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Related Experiment Video

Updated: Mar 11, 2026

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

PrimeRoot: a cutting-edge technology designed to achieve precise and targeted large DNA insertion in plants.

Chandranandani Negi¹, Pritesh Vyas², Raman Dhariwal³

¹Department of Science & Technology, Jayoti Vidyapeeth Women's University, Jaipur, 303122 India.

|March 10, 2026

Summary

PrimeRoot editors precisely insert large DNA fragments up to 11.1 kb into plant genomes, advancing molecular plant breeding and synthetic biology. This genome editing technology offers enhanced precision and efficiency for crop improvement.

Keywords:

CRISPR Genome editing Genomics PegRNA PrimeRoot Site-specific recombinase

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Last Updated: Mar 11, 2026

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

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Published on: April 30, 2016

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

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Published on: March 28, 2018

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

Area of Science:

Plant Science
Molecular Biology
Biotechnology

Background:

Genome editing, particularly CRISPR, revolutionized plant breeding by enabling targeted genomic modifications.
Integrating large DNA segments for enhanced traits remains a significant challenge in plant molecular breeding.

Purpose of the Study:

To introduce PrimeRoot editors as a novel solution for precise chromosomal integration of large DNA inserts in plants.
To review the technology, applications, advantages, and challenges of PrimeRoot editors in plant synthetic biology and breeding.

Main Methods:

Utilizes enhanced prime editing guide RNA (PegRNA) designs.
Incorporates improved plant prime editor systems and advanced recombinases.
Demonstrates precise insertion of DNA fragments up to 11.1 kb.

Main Results:

Achieved precise insertion of large DNA fragments (up to 11.1 kb) into plant genomes.
Third-generation PrimeRoot editors show enhanced precision and efficiency across various gene delivery systems.
Successfully demonstrated applicability in inserting long DNA sequences across different plant species.

Conclusions:

PrimeRoot editors represent a breakthrough for integrating large DNA sequences in plants, crucial for synthetic biology and breeding.
The technology offers significant promise for accelerating crop improvement and developing plants with desired traits.
Further expansion of PrimeRoot editor applicability to diverse plant varieties is expected to drive innovation in agriculture.