Related Experiment Video
Updated: Mar 11, 2026

Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor CAR T Cell Infusions in Mice
Published on: February 24, 2023
Spatial Characteristics of Double Puncture in Dental Anesthetic Cartridges: A Descriptive Morphological Analysis of
Takutoshi Inoue1, Toru Yamamoto2, Naotaka Kishimoto2
1Department of Anatomy, Teikyo University School of Medicine, Tokyo, JPN.
Abstract:
Introduction Puncture of the rubber stopper on dental local anesthetic cartridges is routinely performed during dental practice. Repeated puncture at the same site has been suggested to increase the risk of rubber damage, yet no quantitative evaluation of spatial relationships in double puncture has been reported. This study aimed to characterize the spatial features of double puncture using morphological and spatial indices. Materials and methods One hundred used dental anesthetic cartridges were examined. The first puncture represented routine clinical practice, whereas the second was performed experimentally using a standardized technique. Puncture sites were imaged under a stereomicroscope, and spatial indices, including circle diameter, inclusion rate, and minimum inter-puncture distance, were measured using image superimposition analysis. Results The mean circle diameter was significantly larger at the first puncture than the second (1.59 ± 0.07 vs. 1.31 ± 0.06 mm, p < 0.05). The inclusion rate was 90 ± 3.2%. Minimum distance measurements showed that 33% of cartridges demonstrated inter-puncture proximity of ≤ 0.25 mm. Conclusion Double puncture frequently converges within a restricted area. Spatial overlap may concentrate mechanical stress on the stopper and may represent a potential contributing factor to rubber damage. When an initial puncture fails, replacing the cartridge rather than performing a repuncture may be a reasonable precautionary measure.
More Related Videos
09:08Author Spotlight: Advancing Glioblastoma Treatment Through Intraarterial Delivery Strategies for Oncolytic Viruses and MicroRNAs
Published on: March 15, 2024
10:44Examination of Rapid Dopamine Dynamics with Fast Scan Cyclic Voltammetry During Intra-oral Tastant Administration in Awake Rats
Published on: August 12, 2015
Related Concept Videos
Local Anesthetics: Clinical Application as Surface, Infiltration, and Conduction Block Anesthesia
Local Anesthetics: Pharmacokinetics
Local Anesthetics: Clinical Application as Epidural Anesthesia
Since epidural anesthetics can be infused through an epidural catheter, all types of drugs, including short-acting ones, can be administered. Chloroprocaine and lidocaine are examples of short and long-duration anesthetics, respectively. Bupivacaine...
Local Anesthetics: Clinical Application as Intravenous Regional Anesthesia
One of the advantages of...