Protocol for identifying surface membrane proteins and their associated proteome from mouse cortical neuron cultures...

Modifications to the plasma membrane proteome reflect neuronal physiological states. Here, we present a protocol to profile surface proteins from mouse cortical neuron cultures using in situ biotinylation. The workflow includes neuron culture, surface labeling, enrichment of biotinylated proteins, and on-beads digestion followed by mass spectrometry, enabling quantitative and comparative analysis of membrane-associated proteins across diverse physiological conditions. For complete details on the use and execution of this protocol, please refer to Shi et al.¹.