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Related Concept Videos

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Mitochondria

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Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
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Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
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Asperuloside Protects Against Age-Related Muscle Dysfunction by Enhancing FOXO3-Mediated Mitophagy.

Qi Chen1,2,3, Qinjun Zhang1,3, Jing Wang1,4

  • 1College of Biosystems Engineering and Food Science, Key Laboratory for Quality Evaluation and Health Benefit of Agro-Products of Ministry of Agriculture and Rural Affairs, Key Laboratory for Quality and Safety Risk Assessment of Agro-Products Storage and Preservation of Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China.

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Summary

Asperuloside (ASP) enhances mitophagy and mitochondrial health, alleviating muscle aging phenotypes in cells and mice. This natural compound may prevent age-related muscle disorders by maintaining mitochondrial homeostasis.

Keywords:
FOXO3asperulosideiridoidmitochondriamuscle agingphytochemicals

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Area of Science:

  • Mitochondrial Biology
  • Cellular Aging
  • Pharmacology

Background:

  • Age-related muscle disorders are a growing concern.
  • Mitophagy enhancers offer potential therapeutic strategies.
  • Asperuloside (ASP) is a natural iridoid known to activate mitophagy.

Purpose of the Study:

  • To elucidate the mechanisms of ASP's action on muscle aging.
  • To evaluate ASP's efficacy in preventing age-related muscle decline.
  • To investigate ASP's role in mitochondrial homeostasis and mitophagy.

Main Methods:

  • Senescent myoblast cell models and aging mouse models were used.
  • Techniques included cell staining, western blotting, fluorescence imaging, and Seahorse analysis.
  • Small interfering RNA (siRNA) was employed to study mitophagy regulators.

Main Results:

  • ASP alleviated senescence phenotypes and improved mitochondrial health in senescent myoblasts.
  • ASP induced BNIP3-dependent mitophagy via the FOXO3 transcription factor.
  • ASP supplementation reduced fat accumulation and improved muscle function and mitochondrial morphology in aging mice.

Conclusions:

  • ASP is a potential natural therapeutic agent for preventing muscle aging.
  • BNIP3-dependent mitophagy, modulated by FOXO3, is a key mechanism for ASP's effects.
  • Maintaining mitochondrial homeostasis through mitophagy is crucial for muscle health during aging.