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Updated: Mar 12, 2026

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Smartphones with multispectral imaging for medical testing.

Joseph R Lakowicz1, Kundan Sivashanmugan1, E Albert Reece2

  • 1Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 655 W. Baltimore St, Baltimore MD 21201, United States of America.

Methods and Applications in Fluorescence
|March 10, 2026
PubMed
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Smartphone multispectral imaging (MSI) enhances biomedical diagnostics. MSI smartphones offer improved fluorescence imaging and sensing accuracy over standard RGB cameras, enabling precise biomarker quantification and novel applications.

Area of Science:

  • Biomedical optics
  • Medical imaging technology
  • Smartphone-based diagnostics

Background:

  • Standard red-green-blue (RGB) smartphone cameras have limited spectral resolution, hindering accurate fluorescence imaging and quantitative sensing in biomedical applications.
  • Existing limitations prevent precise differentiation of overlapping spectra and true-color observation of fluorescent biomarkers.

Purpose of the Study:

  • To highlight the advancements and benefits of smartphone-based multispectral imaging (MSI) for biomedical applications.
  • To demonstrate how MSI improves fluorescence imaging and quantitative sensing capabilities compared to traditional RGB imaging.

Main Methods:

  • Utilizing smartphones equipped with multi-channel CMOS sensors and Bragg mirror-based spectral filters for direct multi-wavelength detection.
Keywords:
RGB cameramulti-spectral imagingmulti-wavelengthoptical sensingsmartphone

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  • Comparing the performance of MSI smartphones with RGB smartphones for fluorescence imaging and sensing tasks.

    • Main Results:

    • MSI smartphones achieve enhanced spectral accuracy and enable precise differentiation of overlapping emission spectra.
    • True-color fluorescence observation and quantitative assessment of fluorescent biomarkers are significantly improved.
    • The shift to MSI enhances fluorescence imaging, enabling applications like point-of-care testing and fluorescence-guided surgery.

    Conclusions:

    • Smartphone-based MSI platforms offer compact, user-friendly, and high-resolution solutions for advanced biomedical imaging and optical sensing.
    • These MSI devices significantly enhance the depth and accuracy of fluorescence imaging and sensing in research and clinical settings.
    • MSI technology paves the way for novel diagnostic tools and improved fluorescence-guided interventions.