A dual-mode sensing platform for the detection of glucose based on the Au NPs@Fe-MOF with dual enzyme activity

Increasing evidence links high sugar intake to health issues, highlighting the need for effective glucose sensors in commercial beverages. Here, a dual-ligand metal-organic framework (Fe-MOF) with both electrochemiluminescence (ECL) and peroxidase-like activities was designed, and an enzyme-free glucose sensor was developed using a composite of Fe-MOF and gold nanoparticles with glucose-oxidase activity. The resulting sensor enables quantitative glucose detection via ECL and ultraviolet-visible spectroscopic methods, achieving linear response ranges of 0.001-1 mmol/L and 0.01-1 mmol/L, with limits of detection as low as 0.169 ± 0.001 μmol/L and 2.583 ± 0.046 μmol/L, respectively. Comprehensive experimental validation confirms that the sensor exhibits high repeatability, selectivity, and long-term stability. Moreover, the analysis results of the actual beverage samples were highly consistent with the results of the enzyme-linked colorimetric method, with a relative deviation of less than 2.5%. This work offers significant potential for applications in food quality control and public health-oriented dietary management.