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ESDR/DNAzyme-based signal cascade amplification colorimetric aptamer sensor for detecting fumonisin B1 in cereal
Pan Liu1, Yunzhe Zhang1, Fang Tian2
1College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China.
Abstract:
Fumonisin B1 (FB1) is a water-soluble metabolite produced by various Fusarium species. It poses significant health risks to humans by contaminating cereal-based foods. Therefore, we propose a signal cascade amplified colorimetric aptasensor based on entropy-driven strand displacement reaction (ESDR)/DNAzyme for the rapid, quantitative detection of FB1 in cereal foods. This sensor demonstrates good specificity and repeatability. By using a smartphone to read the color data of the solution, the detection limit is 4.275 ng/mL, with a recovery rate of 89.9-112.88%. By measuring the absorbance, the sensor exhibited a detection limit of 0.14 ng/mL and a recovery rate ranging from 91.65% to 108.5%. This sensor is suitable for on-site detection and use in areas where resources are scarce. The recovery rates of these two methods are essentially the same as those of high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). This provides a new platform for detecting trace amounts of FB1 in food.
