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Advances in Double-Stranded DNA Targeting Technologies.

Zuhao Shen1, Yiqun Liu1, Yingjie Hao1,2

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PubMed
Summary
This summary is machine-generated.

This review explores double-stranded DNA (dsDNA) targeting tools for disease diagnosis and therapy. It covers technologies like CRISPR/Cas and AI integration, highlighting their mechanisms, applications, and future potential.

Keywords:
artificial Intelligencedouble‐stranded DNAgene editinggene targetingin situ Imagingin vitro detection

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • Double-stranded DNA (dsDNA) is crucial for genetic information and disease management.
  • DNA's double helix stability hinders access to internal bases, necessitating advanced targeting tools.

Purpose of the Study:

  • To provide a comprehensive review of current and emerging dsDNA targeting technologies.
  • To analyze the mechanisms, applications, and challenges of these tools in diagnostics and therapeutics.

Main Methods:

  • Review of existing literature on dsDNA targeting tools.
  • Analysis of technologies including hybridization probes, ZFPs, TALENs, CRISPR/Cas, Agos, and λ Exo-pDNA.
  • Exploration of AI integration with molecular tools.

Main Results:

  • Detailed overview of various dsDNA targeting tools and their mechanisms.
  • Highlighting applications in in vitro detection, in situ imaging, and gene editing.
  • Comparison of techniques and discussion of future opportunities and challenges.

Conclusions:

  • dsDNA targeting technologies offer high specificity and structural destabilization for various applications.
  • Integration with AI presents novel opportunities for diagnostics and therapeutics.
  • Addressing challenges is key to translating these innovations into clinical practice.