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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Raman Spectroscopy Instrumentation: Overview01:26

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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Two-Dimensional (2D) NMR: Overview01:12

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The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Noise Management of Surface-Enhanced Raman Spectroscopy Using Two-Dimensional Materials.

Jeewan C Ranasinghe1, Stephen K Sanders1, Ziyang Wang1

  • 1Department of Electrical and Computer Engineering, Rice University, Houston, Texas 77005, United States.

ACS Sensors
|March 11, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed novel hybrid surface-enhanced Raman spectroscopy (SERS) substrates using gold nanoparticles and 2D materials. These new SERS platforms significantly reduce noise and improve signal uniformity for enhanced biomolecular detection.

Keywords:
SERS enhancementbiosensinglimit of detectionraman spectroscopysignal-to-noise ratio (SNR)two-dimensional (2D) materials

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Area of Science:

  • Nanotechnology
  • Spectroscopy
  • Materials Science

Background:

  • Surface-enhanced Raman spectroscopy (SERS) is a sensitive technique for biomolecular detection.
  • SERS performance is often limited by substrate-induced signal non-uniformity and noise.
  • Developing stable and uniform SERS substrates is crucial for reliable biosensing.

Purpose of the Study:

  • To design and evaluate hybrid SERS substrates incorporating gold nanoparticles (AuNP) and two-dimensional (2D) materials for improved noise management.
  • To investigate the impact of 2D materials on spectral uniformity and signal-to-noise ratio (SNR) in SERS.
  • To demonstrate the enhanced biosensing capabilities of these novel hybrid substrates.

Main Methods:

  • Fabrication of hybrid SERS substrates using AuNP and 2D materials (graphene, MoS2, WSe2).
  • Characterization of spectral uniformity and SNR using SERS measurements.
  • Full-wave electromagnetic simulations based on Maxwell's equations to understand noise mechanisms.
  • Demonstration of biosensing using a specific protein biomarker.

Main Results:

  • Hybrid AuNP/2D material substrates exhibited significantly improved spectral uniformity and SNR compared to conventional AuNP substrates.
  • The AuNP/graphene platform demonstrated a ~67% reduction in noise and a ~279% increase in SNR.
  • Simulations revealed that the optical properties of 2D materials and nanoparticle distribution are key factors influencing noise.
  • Enhanced sensitivity was achieved, with a limit of detection (LOD) of 10^-9 M for a receptor binding domain protein using AuNP/graphene.

Conclusions:

  • Hybrid AuNP/2D material substrates offer an effective strategy for noise-managed SERS.
  • These platforms provide enhanced sensitivity and reliability for various biosensing applications.
  • The findings pave the way for advanced SERS-based diagnostics and molecular detection.