Related Experiment Video
Updated: Mar 13, 2026
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
Published on: June 2, 2011
Protocol to annotate dendritic cell maturation types in vivo making use of lipid nanoparticle-based approaches
Karo Van Lil1, Janne Swinnen2, Greta R Webb1
1Laboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
The current panels used to annotate mature dendritic cells (DCs) by flow cytometry do not suffice to deconvolute homeostatic from immunogenic mature DCs. Here, we present a protocol to classify DCs along a homeostatic/immunogenic spectrum making use of flow cytometry and/or transcriptional profiling. We describe steps to steer DC maturation in vivo using a lipid nanoparticle (LNP)-based approach. We detail procedures to formulate, characterize, and generate these LNPs and outline their application in mice. For complete details on the use and execution of this protocol, please refer to Rennen et al.1.
The current panels used to annotate mature dendritic cells (DCs) by flow cytometry do not suffice to deconvolute homeostatic from immunogenic mature DCs. Here, we present a protocol to classify DCs along a homeostatic/immunogenic spectrum making use of flow cytometry and/or transcriptional profiling. We describe steps to steer DC maturation in vivo using a lipid nanoparticle (LNP)-based approach. We detail procedures to formulate, characterize, and generate these LNPs and outline their application in mice. For complete details on the use and execution of this protocol, please refer to Rennen et al.1.
