Related Experiment Video
Updated: Mar 13, 2026
18:30
RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
Published on: February 13, 2013
22.5K
Thrombin Concentration Shapes Endothelial Extracellular Vesicle Profiles with Divergent Inflammatory Functions.
Madhura Chatterjee1, Shiva Keshava2, Prity Dhara1
1BRIC-National Institute of Biomedical Genomics, Kalyani, India.
Blood Advances
|March 11, 2026
Summary
Thrombin
Area of Science:
- Biochemistry
- Cell Biology
- Vascular Biology
Background:
- Thrombin regulates inflammation and vascular barrier function via protease-activated receptor 1.
- Thrombin exhibits concentration-dependent opposing effects: low concentrations are protective, while high concentrations are detrimental.
- Mechanisms underlying thrombin's dual actions remain unclear.
Purpose of the Study:
- To investigate the role of extracellular vesicles (EVs) and their microRNA (miR) cargo in mediating thrombin's concentration-dependent effects on endothelial cells.
- To elucidate the specific miRs involved and their molecular targets in regulating inflammation and vascular integrity.
Main Methods:
- Stimulation of endothelial cells with varying thrombin concentrations to induce EV release.
- Characterization of miR cargo within low- vs. high-thrombin-induced EVs.
- Functional assays using manipulated EVs (e.g., with anti-miRs or miR mimics) to confirm causal roles.
- Analysis of target gene regulation (USP7, SOCS1) and downstream signaling pathways (NF-kB, cytokine signaling).
Main Results:
- Thrombin stimulates EV release, with distinct miR profiles at low and high concentrations.
- Low-thrombin EVs deliver miR-409-5p, targeting USP7 to confer anti-inflammatory and barrier-protective effects.
- High-thrombin EVs deliver miR-155-5p, targeting SOCS1 to promote inflammation and barrier disruption.
- Experimental manipulation of these miRs confirmed their causal roles in mediating thrombin's opposing effects.
Conclusions:
- EV-associated miRs are key mediators of thrombin's concentration-dependent dual actions on vascular cells.
- miR-409-5p and miR-155-5p represent critical regulators of thrombin-induced inflammation and barrier function.
- Engineered EVs delivering specific miRs or anti-miRs hold therapeutic potential for inflammatory vascular diseases.
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