Related Experiment Video
Updated: Mar 13, 2026
11:56
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
Published on: September 28, 2017
10.5K
NAD+ controls circadian rhythmicity during cardiac aging
Bryce J Carpenter1,2,3, Margaux Lecacheur1,2,3, Yannick N Mangold1,3
1Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Communications Biology
|March 12, 2026
Summary
Aging disrupts the heart's daily rhythms and lowers NAD+. Supplementing with nicotinamide riboside (NR) restores NAD+ levels, resets the cardiac clock, and reverses age-related heart enlargement in mice.
Area of Science:
- Gerontology and Cardiovascular Science
- Circadian Biology and Metabolism
Background:
- Aging is characterized by circadian clock disruption and decreased NAD+ levels.
- The interplay between circadian rhythms and NAD+ metabolism in cardiac aging remains poorly understood.
Purpose of the Study:
- To investigate the relationship between cardiac circadian rhythms and NAD+ metabolism during aging.
- To determine the effects of NAD+ precursor supplementation on cardiac aging.
Main Methods:
- Analysis of diurnal gene expression in the aging mouse heart.
- Long-term supplementation with nicotinamide riboside (NR) in aged female mice.
- Assessment of NAD+ levels and PER2::luc oscillations in cardiomyocytes.
- Investigation of SIRT1 activity in response to NR treatment.
Main Results:
- Aging disrupts diurnal gene expression patterns in the heart.
- NR supplementation restored NAD+ levels, reprogrammed the cardiac transcriptome, and reversed cardiac enlargement in aged mice.
- Reduced NAD+ impaired cardiomyocyte circadian oscillations, which were rescued by NR.
- NR-induced transcriptomic changes were partially dependent on SIRT1 activity.
Conclusions:
- NAD+ plays a critical role in regulating the cardiac circadian clock during aging.
- NR supplementation offers a potential therapeutic strategy to counteract age-related cardiac dysfunction.
- This research highlights novel avenues for addressing cardiovascular disorders associated with aging.
More Related Videos
Related Concept Videos
02:19Circadian Rhythms and Gene Regulation
4.7K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.7K
02:19Circadian Rhythms and Gene Regulation
2.4K
2.4K
01:15Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response
436
Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...
436
01:31Regulation of Heart Rates
4.4K
The regulation of heart rate is a complex process controlled by the autonomic nervous system (ANS), hormonal influences, and intrinsic cardiac mechanisms. The ANS has two main components: the sympathetic nervous system (SNS) and the parasympathetic nervous system (PNS).
The SNS increases heart rate through the release of norepinephrine and epinephrine, which act on beta-1 adrenergic receptors in the heart. This action increases the rate of depolarization in the sinoatrial (SA) node, the heart's...
The SNS increases heart rate through the release of norepinephrine and epinephrine, which act on beta-1 adrenergic receptors in the heart. This action increases the rate of depolarization in the sinoatrial (SA) node, the heart's...
4.4K
01:28Mechanism of Cardiac Arrhythmias
2.5K
Arrhythmias are irregular heart rhythms occurring when the heart's electrical impulses become abnormal. These disturbances can lead to various symptoms, depending on their severity and the underlying cause. Some common factors contributing to arrhythmias include hypoxia, ischemia, electrolyte imbalances, excessive catecholamine exposure, drug toxicity, and muscle overstretching. Arrhythmias can be classified into two main types based on the rate and site of origin of abnormal heart rhythms.
2.5K
01:19Electrophysiology of Normal Cardiac Rhythm
10.0K
The normal cardiac rhythm is a synchronized electrical activity that facilitates the regular and coordinated contraction of the heart muscle. This process is essential for efficient blood circulation throughout the body. The fundamental elements involved in establishing and maintaining this rhythm include the unique electrical properties of cardiac muscle cells, the sinoatrial (SA) node's pacemaker function, the specialized conducting system, and the ionic mechanisms underlying each phase...
10.0K