Adaptive ROS-Responsive Dual-Layered Hydrogel with Ginkgo Biloba-Derived Exosome-Like Nanovesicles for Diabetic Wound...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Wound Healing Research

Background:

• Diabetic wound healing is hindered by infection and imbalanced reactive oxygen species (ROS).
• Existing hydrogel dressings struggle to address the distinct needs of different wound healing stages.
• A dynamic approach to ROS regulation is crucial for effective diabetic wound management.

Purpose of the Study:

• To develop a multifunctional bilayer hydrogel (Dual-Gel) for diabetic wound healing.
• To engineer a system that precisely regulates dynamic ROS levels in the wound microenvironment.
• To create a smart dressing that responds to wound conditions for improved healing outcomes.

Main Methods:

• Synthesized a bilayer hydrogel: Gel 1 (EPLGMA/copper ions) and Gel 2 (gelatin/ROS-cleavable linker).
• Gel 1 provides antibacterial activity and generates ROS via copper ion catalysis.
• Gel 2 degrades upon excessive ROS, releasing exosomes to promote healing.

Main Results:

• The Dual-Gel system effectively managed infection and regulated ROS levels.
• Gel 1 released ε-polylysine for antibacterial action and copper ions for ROS generation.
• Gel 2 degradation released exosomes, stimulating cell proliferation, angiogenesis, and anti-apoptosis.

Conclusions:

• The Dual-Gel system offers a novel strategy for diabetic wound management by dynamically controlling ROS.
• This smart hydrogel dressing orchestrates the release of bioactive components in response to wound microenvironment changes.
• The Dual-Gel system shows potential for improving clinical outcomes in complex diabetic wounds.