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Related Experiment Video

Updated: Mar 13, 2026

Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
09:43

Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts

Published on: August 1, 2025

937

Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants.

Giulia Gangarossa1, Caterina Grillo1, Sara Roccabianca1

  • 1Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134, Florence, Italy.

Bio-Protocol
|March 12, 2026
PubMed
Summary

Researchers developed a new method to isolate cancer-associated fibroblasts (CAFs) from prostate cancer tissue. This technique enables better study of how these cells influence tumor progression and interactions within the tumor microenvironment.

Keywords:
Cancer-associated fibroblastsExplant cultureFibroblast activationPrimary culturesProstate cancer

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Area of Science:

  • Oncology
  • Cell Biology
  • Biotechnology

Background:

  • Prostate carcinoma (PCa) progression is significantly impacted by its tumor microenvironment.
  • Cancer-associated fibroblasts (CAFs) are a key stromal component influencing PCa.
  • Limited availability of stable CAF models hinders research into stromal-tumor interactions.

Purpose of the Study:

  • To establish an optimized protocol for isolating cancer-associated fibroblasts (CAFs) from fresh human prostate cancer biopsies.
  • To provide a reliable ex vivo model for investigating CAF biology and their role in the tumor microenvironment.

Main Methods:

  • A mechanical isolation procedure was employed, leveraging the migratory properties of CAFs from tumor explants.
  • The protocol avoids enzymatic treatments to minimize stress and preserve fibroblast phenotype.
  • Selective outgrowth of CAFs from prostate tumor explants was utilized for isolation.

Main Results:

  • An optimized workflow for isolating CAFs from prostate tumor explants was successfully developed.
  • The enzymatic-free method preserves tissue architecture, CAF viability, and phenotypic characteristics.
  • The resulting ex vivo CAF cultures serve as a suitable model for studying stromal-tumor interactions.

Conclusions:

  • This optimized protocol facilitates the isolation of functional CAFs from PCa biopsies.
  • The ex vivo CAF model is valuable for advancing the understanding of prostate cancer progression.
  • The method supports research into the complex interactions within the tumor microenvironment.