Casein-liposome complex loading basil essential oil: Preparation, antibacterial effect, and preservative effect on...

Liposomal encapsulation enhances the stability of essential oils, while simple liposomes are plagued by lack of targeting and low release rate. Herein, bacteria-secreted protease-triggered liposomes (BEO-clip) were prepared from basil essential oil-loaded liposomes (BEO-lip). The physiochemical properties, antibacterial effect and mechanism against Staphylococcus aureus of the prepared liposomes were investigated, the liposomes were also utilized for the preservation of braised beef. BEO-clip presented higher storage and thermal stabilities. In addition to a stronger bactericidal effect against S. aureus, BEO-clip exhibited a bacteria-secreted protease-dependent BEO release and sustained antibacterial effects. BEO-clip showed a more intense interaction with S. aureus, causing membrane disruption, declined membrane potential, and increased surface charge. Both BEO-lip and BEO-clip effectively inhibited the microbial growth in braised beef during storage. BEO-clip significantly restrained lipid oxidation, suppressed the increase of pH and total volatile base nitrogen, delayed the deterioration of color and texture, without comprising sensory quality.