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UV–Vis Spectrometers01:14

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Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
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Evaluation of Tinted Sunscreens Protection Against Blue Light Using Transmittance Spectroscopy In Vitro Technique.

Hawasatu Dumbuya1, Katharine Podimatis1, Pearl Grimes2

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|March 13, 2026
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Summary

Tinted sunscreens offer varied protection against high energy visible (HEV) light. Formulations with iron oxide and inorganic filters provide superior blue light blocking, highlighting the need for standardized testing methods.

Keywords:
blue light protectionhigh energy visible lightiron oxidestinted sunscreens

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Area of Science:

  • Dermatology and photobiology
  • Cosmetic science
  • Materials science

Background:

  • High energy visible (HEV) light, a component of the visible light spectrum (415-455 nm), possesses significant photobiological effects on skin.
  • Current regulatory guidelines for assessing sunscreen protection against HEV light are lacking.
  • Tinted sunscreens are increasingly popular, but their efficacy against HEV light is not well-established.

Purpose of the Study:

  • To evaluate the in vitro HEV-blocking potential of various commercialized tinted sunscreens.
  • To assess the impact of different formulations, including UV filter systems, antioxidants, and iron oxide (FeO) concentrations, on HEV protection.
  • To determine how blue light irradiation affects the HEV-blocking capabilities of these sunscreens.

Main Methods:

  • Commercialized tinted sunscreens (SPF 30-50) with varying formulations were tested.
  • HEV transmittance (415-455 nm) was measured using transmittance spectroscopy before and after blue light irradiation (200 J/cm²).
  • HEV-blocking potential was calculated based on transmittance measurements.

Main Results:

  • Sunscreens containing iron oxide (FeO) and both zinc oxide and titanium dioxide showed higher HEV absorbance and blocking potential.
  • Higher FeO concentrations correlated with increased HEV absorbance and blocking efficacy.
  • Formulations with organic filters or hybrid systems demonstrated lower HEV protection compared to those with FeO and inorganic filters.

Conclusions:

  • Commercial tinted sunscreens exhibit variable protection against HEV/blue light, influenced by their formulation.
  • The concentration of iron oxide is a key factor in enhancing HEV-blocking properties.
  • Further research and standardized methods are necessary to accurately measure the efficacy of sunscreens against visible/blue light exposure.