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Multilayered Transcriptional Regulation Underlying Salt Tolerance in Rapeseed (Brassica napus L.) Revealed by
Sana Basharat1,2, Hafiza Amina Iqbal1,3, Latif Ullah Khan1,2
1School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), College of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, China.
Biology
|March 13, 2026
Summary
Soil salinity hinders rapeseed (Brassica napus) growth. This study reveals how rapeseed responds to salt stress by identifying key molecular networks, offering targets for improving salt-tolerant rapeseed varieties.
Area of Science:
- Plant Biology
- Molecular Genetics
- Agricultural Science
Background:
- Soil salinity is a major abiotic stress limiting crop productivity worldwide.
- Rapeseed (Brassica napus) is susceptible to salinity, impacting its yield and cultivation range.
- Understanding the molecular mechanisms of salt tolerance is crucial for crop improvement.
Purpose of the Study:
- To investigate the physiological, biochemical, and transcriptomic responses of Brassica napus to salt stress.
- To identify key long non-coding RNA (lncRNA)-mRNA regulatory networks involved in salinity tolerance.
- To uncover molecular targets for enhancing salt resilience in rapeseed.
Main Methods:
- Controlled salt stress experiment (100 mM NaCl) on Brassica napus inbred line 383-5.
- Physiological and biochemical assays to assess growth, oxidative damage, and osmotic adjustment.
- Genome-wide transcriptomic analysis (RNA-Seq) to identify differentially expressed genes and lncRNAs.
- Bioinformatic analyses for functional enrichment and lncRNA-mRNA network construction.
Main Results:
- Salt stress caused significant growth inhibition, oxidative damage, and osmotic adjustment in rapeseed.
- Extensive transcriptional reprogramming was observed, with 6215 protein-coding genes and 941 lncRNAs identified as salt-responsive.
- Key pathways involved include ion transport, redox homeostasis, phytohormone signaling (ABA, ethylene), and secondary metabolism (phenylpropanoid, lignin biosynthesis).
- lncRNA-mRNA networks revealed non-coding RNAs modulating hormone signaling, redox balance, and metabolic adaptation.
Conclusions:
- Brassica napus exhibits a complex, multilayered regulatory framework for salinity tolerance involving coordinated physiological and molecular responses.
- Phytohormone signaling, antioxidant systems, and cell wall remodeling are critical components of salt stress adaptation.
- Identified lncRNAs and their regulatory networks provide valuable targets for breeding salt-resilient rapeseed cultivars.
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