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A Fluorescent Aptasensor Based on Tb-MOFs and Its Application for the Detection of Pseudomonas aeruginosa in Foods
Jinqiong Xu1, Xinyu Yu2, Jianbo Liu1
1College of Chemistry and Chemical Engineering, Xianyang Normal University, Xianyang 712000, China.
Abstract:
Pseudomonas aeruginosa is an important waterborne pathogen that is harmful to food safety and human health. Therefore, the detection of Pseudomonas aeruginosa has become an essential item in food sampling. This article constructs a fluorescent aptamer sensor based on Tb-MOFs, using Tb-MOFs as the fluorescence signal source, colloidal gold (AuNPs) as the signal conversion switch, and aptamers as the target recognition element, to establish a quantitative detection method for Pseudomonas aeruginosa. The constructed sensor exhibits a good linear relationship within the concentration range of Pseudomonas aeruginosa from 1 to 106 CFU/mL, with a detection limit of 0.63 CFU/mL. Moreover, the sensor was also applied to the detection of Pseudomonas aeruginosa in actual samples of bottled drinking water and orange juice. The fluorescent aptasensor based on Tb-MOFs provides a rapid and sensitive new sensing method for the detection of Pseudomonas aeruginosa in food.
Insights
This study presents a new fluorescent aptamer sensor for detecting Pseudomonas aeruginosa, a harmful waterborne pathogen. The sensor offers a rapid and sensitive method for identifying this bacterium in food and water samples.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Materials Science
Background:
- Pseudomonas aeruginosa is a significant waterborne pathogen impacting food safety and human health.
- Accurate detection of Pseudomonas aeruginosa is crucial for food surveillance.
- Existing detection methods may lack sensitivity or speed for routine food analysis.
Purpose of the Study:
- To develop a novel fluorescent aptamer sensor for the quantitative detection of Pseudomonas aeruginosa.
- To utilize terbium-based metal-organic frameworks (Tb-MOFs) as a fluorescence source.
- To create a sensitive and rapid detection platform for food safety applications.
Main Methods:
- Construction of a fluorescent aptamer sensor using Tb-MOFs, colloidal gold nanoparticles (AuNPs), and specific aptamers.
- Tb-MOFs served as the fluorescence signal generator.
- AuNPs acted as a signal conversion switch for quantitative analysis.
- Aptamers were employed for specific recognition of Pseudomonas aeruginosa.
Main Results:
- The sensor demonstrated a good linear relationship for Pseudomonas aeruginosa concentrations ranging from 1 to 10^6 CFU/mL.
- A low detection limit of 0.63 CFU/mL was achieved.
- The sensor successfully detected Pseudomonas aeruginosa in real-world samples, including bottled drinking water and orange juice.
Conclusions:
- The developed Tb-MOF-based fluorescent aptasensor provides a novel, rapid, and sensitive method for Pseudomonas aeruginosa detection.
- This sensor holds promise for routine monitoring of Pseudomonas aeruginosa in the food industry.
- The study highlights the potential of MOF-based aptasensors in food safety diagnostics.
