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Related Experiment Video

Updated: May 7, 2026

The MultiBac Protein Complex Production Platform at the EMBL

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Published on: July 11, 2013

Emerging technologies for intensifying functional single-cell ingredient production.

Khalilan Lambangsari¹, Oscar M Elizondo Sada², Inge I A Braak²

¹Bioprocess Engineering, Wageningen University & Research, Wageningen, The Netherlands; School of Life Sciences and Technology, Bandung Institute of Technology, Bandung, Indonesia.

Trends in Biotechnology

|March 15, 2026

Summary

New downstream processing methods using external fields, cold plasma, and supercritical fluids can improve microbial biotechnology. This review explores these advanced techniques for more sustainable and efficient production of functional ingredients from microbial and microalgal biorefineries.

Keywords:

external fields mild processing multiproduct biorefinery process intensification single-cell ingredient

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Last Updated: May 7, 2026

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Area of Science:

Microbial biotechnology and bioprocessing.

Background:

Microbial biotechnology yields valuable compounds for food, feed, nutraceutical, therapeutic, and cosmetic industries.
Current extraction and purification methods are often harsh, costly, and environmentally burdensome, hindering efficient production.
A paradigm shift in downstream processing is essential for advancing microbial and microalgal biorefineries.

Purpose of the Study:

To review and analyze the potential and limitations of novel downstream processing technologies.
To propose strategies for overcoming current challenges in biomass processing.
To guide the development of next-generation multiproduct microbial and microalgal biorefineries.

Main Methods:

Review of literature on external fields, cold plasma, and supercritical fluid technologies for biomass processing.
Analysis of the strengths and weaknesses of these emerging techniques.
Identification of opportunities for process simplification and intensification.

Main Results:

External fields, cold plasma, and supercritical fluids offer promising avenues for simplified and intensified biomass processing.
These technologies have the potential to reduce economic and environmental costs associated with compound extraction and purification.
A comprehensive understanding of their specific advantages and disadvantages is still developing.

Conclusions:

Novel downstream processing technologies are crucial for unlocking the full potential of microbial and microalgal biotechnology.
Further research and strategic implementation are needed to overcome existing limitations and optimize these methods.
Advancements in these areas will pave the way for more sustainable and efficient multiproduct biorefineries.