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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Related Experiment Video

Updated: Mar 17, 2026

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography
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GAS5 Long Noncoding RNA Regulates CD20 Expression and Rituximab Response.

Mahbubeh Rojhannezhad1, Zahra Abedi Kichi1,2, Abbas Nikravesh3

  • 1Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Advanced Pharmaceutical Bulletin
|March 16, 2026
PubMed
Summary

Growth arrest specific 5 (GAS5) long non-coding RNA influences rituximab effectiveness in non-Hodgkin lymphoma by indirectly regulating CD20 expression. Modulating GAS5 enhances rituximab

Keywords:
ApoptosisB-cell lymphomaCD20GAS5Rituximab response

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Rituximab is a key therapy for non-Hodgkin lymphoma (NHL).
  • Therapeutic resistance to rituximab, often due to CD20 downregulation, limits its effectiveness.
  • Long non-coding RNAs (lncRNAs), such as GAS5, are implicated in cancer drug response.

Purpose of the Study:

  • To investigate the regulatory role of GAS5 on CD20 expression.
  • To determine GAS5's effect on cancer cell response to rituximab.

Main Methods:

  • Utilized the Raji cell model for experiments.
  • Assessed GAS5 knockdown effects on CD20 expression via RT-qPCR.
  • Evaluated protein expression, apoptosis, and oxidative stress using Western blot, caspase-3 activity, and ROS assays.

Main Results:

  • GAS5 knockdown altered CD20, STAT3, and SMAD2 expression, impacting apoptosis and autophagy.
  • GAS5 knockdown reduced reactive oxygen species (ROS) generation.
  • Combined GAS5 knockdown and rituximab treatment increased apoptosis and autophagy while decreasing ROS.

Conclusions:

  • GAS5 appears to indirectly regulate CD20 expression through associated proteins.
  • Understanding the GAS5-CD20 interaction is crucial for improving rituximab efficacy in NHL.
  • Further research is needed to explore these complex interactions in cancer therapy.