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Updated: Jun 19, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Modular Scaffold Crystals for Programmable Installation and Structural Observation of DNA-Binding Proteins.

Ethan T Shields¹, Caroline K Slaughter², Fadwa Mekkaoui³

¹School of Biomedical and Chemical Engineering, Colorado State University; Fort Collins, 80523, United States.

Biorxiv : the Preprint Server for Biology

|March 16, 2026

Summary

Researchers developed a novel protein-DNA co-crystal for biomacromolecule crystallization. This method simplifies structure determination by decoupling crystal growth from guest molecule installation, enabling high-throughput analysis.

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$On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature$

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

Area of Science:

Structural Biology
Biochemistry
Materials Science

Background:

Biomacromolecule crystallization for structural studies is challenging, often requiring extensive experimental screening.
Existing methods struggle with organizing diverse guest molecules for diffraction-quality crystal formation.

Purpose of the Study:

To develop a novel protein-DNA co-crystal system for efficient and high-throughput biomacromolecule crystallization.
To enable precise control over guest molecule positioning within the crystal lattice.

Main Methods:

Engineered protein-DNA co-crystals composed of stacked DNA scaffolds and protein columns.
Designed solvent channels for guest protein diffusion and installation.
Utilized DNA strut variation for positionally controlled guest protein binding.

Main Results:

Demonstrated successful co-crystal formation with tunable DNA scaffolds.
Achieved sub-nanometer control over guest macromolecule position and orientation.
Established a method decoupling scaffold crystal growth from guest molecule soaking.

Conclusions:

The protein-DNA co-crystal system facilitates high-throughput structure determination of DNA-binding proteins and conjugates.
This approach overcomes limitations of traditional crystallization screening.
Offers potential for functional applications beyond structural biology.