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Updated: Mar 17, 2026
Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
Published on: March 5, 2021
3D positioning and optically detected magnetic resonance of intracellular fluorescent nanodiamonds using a
Bowen Zhang1,2, Yichen Yang1,2, Jia Su1,2
1CAS Key Laboratory of Microscale Magnetic Resonance and School of Physical Sciences, University of Science and Technology of China, Hefei 230026, China.
Abstract:
Wide-field quantum sensing with fluorescent nanodiamonds (FNDs) in biological systems offers significant potential for understanding intracellular dynamics at the nanoscale. However, current wide-field quantum sensing methods are limited to 2D correlated measurements. 3D correlated quantum sensing remains challenging due to the inherent properties of wide-field microscopy. Here, we have developed a multi-plane wide-field microscope platform that achieves an imaging volume of 50 × 50 × 5 μm³. This is accomplished by simultaneously imaging eight focal planes at varying sample depths using a beam-splitting prism. By employing a Fourier-transform-based fluorescent particle positioning method, the platform attains lateral positioning precision of 9 nm and axial precision of 12 nm. Using this platform, we performed correlated 3D positioning of FNDs in mouse cardiomyocytes and conducted optically detected magnetic resonance on nitrogen-vacancy color centers within intracellular FNDs. Our results demonstrate the potential of this platform for single-particle tracking and highlight its capability to achieve correlated 3D quantum sensing.
