Related Experiment Video

Updated: Mar 18, 2026

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
09:32

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

15.7K

IL20RB promotes proliferation and migration in clear cell renal cell carcinoma and is associated with immune

Yufeng Liu1, Yingmin Xie1, Lingfei Yan1

  • 1The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China.

Peerj
|March 16, 2026
PubMed
Abstract

Insights

Interleukin 20 receptor subunit beta (IL20RB) is downregulated in clear cell renal cell carcinoma (ccRCC) and linked to poor survival. IL20RB expression influences immune cell infiltration and ccRCC cell behavior, suggesting its therapeutic potential.

Area of Science:

  • Oncology
  • Immunology
  • Genetics

Background:

  • Interleukin 20 receptor subunit beta (IL20RB) is a cytokine receptor subunit with a known role in human malignancies.
  • The specific association between IL20RB expression, clinical outcomes, and tumor-infiltrating lymphocytes in clear cell renal cell carcinoma (ccRCC) remains largely uncharacterized.

Purpose of the Study:

  • To investigate the role of IL20RB in ccRCC.
  • To determine the correlation between IL20RB expression, clinicopathological features, and patient prognosis.
  • To explore the relationship between IL20RB and the tumor immune microenvironment in ccRCC.

Main Methods:

  • Utilized The Cancer Genome Atlas (TCGA) for gene expression and clinicopathological data analysis.
  • Performed survival analysis using Kaplan-Meier curves.
  • Analyzed protein-protein interactions, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways.
  • Assessed tumor immune infiltration using ssGSEA, TIMER, and TISIDB databases.
  • Validated IL20RB expression and function through immunohistochemistry (IHC), Western blot, CCK-8, colony formation, wound healing, and Transwell assays.

Main Results:

  • IL20RB expression was significantly lower in ccRCC tissues compared to adjacent normal tissues.
  • Reduced IL20RB expression correlated with advanced tumor classification, lymph node metastasis, poor differentiation, and disease progression.
  • Higher IL20RB expression was associated with unfavorable Disease-Specific Survival (DSS) and Overall Survival (OS) (p < 0.01).
  • IL20RB overexpression linked to increased immunomodulators, chemokines, and infiltration of regulatory T cells (Treg), NK CD56 cells, Th1 cells, cytotoxic cells, and T helper cells.
  • IL20RB knockdown suppressed ccRCC cell proliferation and migration.

Conclusions:

  • Elevated IL20RB expression in ccRCC is associated with a poorer prognosis and increased immune cell infiltration.
  • IL20RB may serve as a potential biomarker for predicting ccRCC patient outcomes.
  • Targeting IL20RB could represent a promising strategy for ccRCC immunotherapy development.
Keywords:
Clear cell renal cell carcinomaIL20RBPrognosisTumor immune microenvironment

More Related Videos

A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies06:38

A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies

Published on: April 12, 2017

14.4K
Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane05:36

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

9.6K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.9K
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
3.1K
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
4.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K