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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
ERGA-BGE Chromosome-Level Genome of Patella rustica Linnaeus, 1758: a Resource to Investigate Responses to Global
Rui Faria1,2,3, Rocío Nieto Vilela1,2, Fernando P Lima1,2
1CIBIO, InBIO Laboratório Associado, Universidade do Porto Centro de Investigacao em Biodiversidade e Recursos Geneticos, Vairão, Porto, Portugal.
Abstract:
The reference genome of Patella rustica Linnaeus, 1758 (Gastropoda, Patellidae) will offer a unique opportunity to understand how intertidal organisms respond to the effects of global warming, contributing to improve our predictions about the impact of climate change on the maintenance and diversification of marine biodiversity. A total of 9 contiguous chromosomal pseudomolecules were assembled from the genome sequence, made up from Oxford Nanopore Technologies (ONT) long-reads, WGS and Chromatin-Conformation-Capture (Hi-C) short-read sequences. This chromosome-level assembly encompasses 719 Mb, composed of 157 contigs and 24 scaffolds, with contig and scaffold N50 values of 8.52 Mb and 83.6 Mb, respectively, and assembly and annotation BUSCO completeness of 97.7% and 96.5%, respectively. The production of this genome assembly and annotation was coordinated and supported by the European Reference Genome Atlas (ERGA) and Biodiversity Genomics Europe project (BGE).
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