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Related Experiment Video

Updated: Mar 18, 2026

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize
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Integrated Single-Cell and Spatial Transcriptomics Reveal Cell-Type-Specific Immune Regulatory Networks in Maize

Qiongqiong Wang1,2, Xinyan Sun1, Yingchao Sun2

  • 1Institute of Cereal Crops, Henan Academy of Agricultural Sciences, The Shennong Laboratory, Zhengzhou, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|March 16, 2026
PubMed
Summary

Southern corn rust threatens maize. This study reveals cell-specific defenses, identifying ZmXET1 as a susceptibility factor and ZmRBG as a resistance component for improved breeding.

Keywords:
Puccinia polysora‐maize interactioncell‐cell communicationimmune regulatory networkssnRNA‐seqstRNA‐seq

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Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Genomics

Background:

  • Southern corn rust (SCR), caused by Puccinia polysora, significantly impacts maize yield.
  • Understanding cell-type-specific defense mechanisms against SCR is crucial for developing resistant varieties.

Purpose of the Study:

  • To investigate the cell-type-specific transcriptional dynamics in maize leaves during early P. polysora infection.
  • To identify key genes and pathways involved in maize defense against Southern corn rust.

Main Methods:

  • Integration of single-nucleus RNA sequencing (snRNA-seq) and spatial transcriptomic sequencing (stRNA-seq).
  • Analysis of transcriptional changes at 24 and 48 hours post-infection (hpi).
  • Virus-induced gene silencing (VIGS) for functional gene validation.

Main Results:

  • Eight major cell types identified with defense responses primarily in mesophyll and epidermal cells at 24 hpi.
  • Cell-type-specific activation of receptor-like kinases/proteins (RLKs/RLPs) and jasmonic acid pathways observed.
  • ZmXET1 identified as a susceptibility factor, while ZmRBG acts as a resistance component.

Conclusions:

  • Provides a high-resolution spatiotemporal atlas of maize defense against P. polysora.
  • Identifies ZmXET1 and ZmRBG as valuable targets for breeding disease-resistant maize.
  • Elucidates cell-type-specific immune responses in maize leaves during pathogen attack.