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Generation of a Mouse Prostate Organoid-Based Model for Studying Host-Pathogen Interactions
Maria Guedes1, Janina Rieger1, Carmen Aguilar2
1Host Pathways in Urinary Tract Infections Group, Institute of Molecular Infection Biology, University of Würzburg.
Journal of Visualized Experiments : Jove
|March 16, 2026
Summary
Researchers developed a new 2D prostate culture model from organoids. This accessible model aids in studying prostate diseases and host-pathogen interactions, offering a valuable tool for therapeutic development.
Area of Science:
- Urology
- Cell Biology
- Infectious Disease
Background:
- Prostate diseases significantly impact aging men's health.
- Organoid models offer in vitro replication of prostate epithelial features.
- Limitations in organoid models hinder apical surface access for certain studies.
Purpose of the Study:
- To develop a 2D prostate culture system from mouse organoids.
- To enable direct apical surface access for biological and interaction studies.
- To create a model for investigating prostate diseases and host-pathogen interactions.
Main Methods:
- Dissociation of mature mouse prostate organoids.
- Seeding cells in a 2D culture system.
- Immunofluorescence microscopy, TEER measurements, and F-actin/tight junction staining.
- Infection with uropathogenic Escherichia coli (UPEC).
Main Results:
- The 2D model maintains prostate epithelial characteristics and cellular organization.
- Confirmed expression of prostate-specific markers.
- Demonstrated cell differentiation and barrier formation.
- Successfully modeled host-pathogen interactions with UPEC.
Conclusions:
- The 2D organoid-derived model provides a physiologically relevant platform.
- Facilitates research into prostate epithelial biology and disease mechanisms.
- Offers a valuable system for studying host-pathogen interactions and testing therapies.
