Automated HPLC Separation Using LC-mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale
1Natural Product Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences; University of Chinese Academy of Sciences.
Journal of Visualized Experiments : Jove
|March 16, 2026
Summary
This study introduces LC-mate, an automated system for high-performance liquid chromatography (HPLC) purification. It enhances efficiency and reproducibility by automating sample injection and fraction collection, reducing manual labor in chemical and biological separations.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
Background:
- High-performance liquid chromatography (HPLC) is vital for purifying valuable compounds in chemistry and biology.
- Manual injection and fraction collection in microscale HPLC limit efficiency and reproducibility.
Purpose of the Study:
- To develop an automated system for repetitive HPLC separations.
- To overcome the limitations of manual sample handling in HPLC purification.
Main Methods:
- An integrated autosampler and fraction collector (LC-mate) was developed.
- The system uses a six-port switching valve for injection and a selector valve for fraction collection.
- Operational parameters are controlled via a touchscreen and triggered by HPLC detector analog signals.
Main Results:
- LC-mate demonstrated stable operation and reproducible separations for natural product purification.
- The system significantly reduced manual handling time.
- It is compatible with commercial HPLC detectors with analog output.
Conclusions:
- LC-mate provides a practical solution for automating conventional HPLC workflows.
- The system bridges the gap between manual and fully automated purification methods.
- It enhances efficiency and reproducibility in microscale HPLC purification.
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