Screening of a xylanase-producing Trichoderma strain and optimization of its enzyme production conditions

Xylanases have attracted considerable attention due to their strong potential for industrial use. In this study, a xylanase-producing strain isolated from soil was identified as Trichoderma semiorbis Tsejk8, and the conditions for xylanase production were optimized. Additionally, two xylanase-related genes were cloned, and their functions were analyzed. The optimal conditions for xylanase production included maltose as the carbon source, peptone as the nitrogen source, an optimal pH of 6.0, and an incubation time of 120 h, yielding an enzyme activity of 40.7 U/mL. Following the purification of xylanase via ammonium sulfate precipitation and ion exchange chromatography, four distinct protein bands were observed. Mass spectrometry analysis of these bands identified 14 associated proteins. Bioinformatics analysis revealed that two of these proteins belong to GH3 (Glycoside Hydrolase family 3) β-xylosidase. In summary, the newly isolated strain Tsejk8 exhibits xylanase activity, offering an effective and eco-friendly means of converting biomass into raw materials for industrial applications.