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Fungicide Sensitivity Profiles of Botrytis Isolates from Blueberry and Red Raspberry Fields in Washington and Oregon
Roshani Baral1, Jeffery A DeLong2, Mary Steele3
1University of Nebraska-Lincoln College of Agricultural Sciences and Natural Resources, Department of Plant Pathology, 1875 N 38th St, Lincoln, Nebraska, United States, 68583; rbaral2@huskers.unl.edu.
Plant Disease
|March 17, 2026
Summary
Fungicide resistance in Botrytis species is a growing concern for small fruit production. Monitoring these resistance profiles is crucial for effective disease management in blueberries and raspberries.
Area of Science:
- Plant Pathology
- Agricultural Entomology
- Mycology
Background:
- * Botrytis species cause significant economic losses in U.S. blueberry and red raspberry production.
- * Conventional management relies on repeated fungicide applications, increasing selection pressure for resistance.
- * Understanding fungicide resistance profiles is vital for sustainable small fruit cultivation.
Purpose of the Study:
- * To monitor the fungicide resistance profiles of Botrytis species affecting blueberries and red raspberries in Washington and Oregon.
- * To assess resistance frequencies to various Fungicide Resistance Action Committee (FRAC) groups.
- * To identify potential cross-resistance patterns.
Main Methods:
- * 708 and 305 Botrytis isolates were collected from blueberry and red raspberry fields in 2022 and 2023.
- * In vitro conidial germ tube growth assays were used to screen isolates against technical grade fungicides.
- * Isolates were tested against specific fungicides from FRAC groups 7, 9, 11, and 17.
Main Results:
- * High resistance frequencies were observed in blueberry isolates to boscalid (60%), fluopyram (49%), fluxapyroxad (38%), and pyraclostrobin (68%).
- * Red raspberry isolates showed the highest resistance to fluopyram (79%) and pyrimethanil (31%).
- * Prevalent cross-resistance was noted within FRAC group 11 and for certain fungicide combinations in FRAC group 7.
Conclusions:
- * Unique fungicide resistance patterns in Botrytis spp. were identified in Pacific Northwest small fruits.
- * Ongoing monitoring of fungicide resistance is essential for effective disease control strategies.
- * Findings underscore the need for adaptive management practices to combat fungicide resistance.

