Related Experiment Video

Updated: Mar 19, 2026

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
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Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

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DNA Methylation-Regulated FAM107A Affects Colorectal Carcinogenesis by Inhibiting FOXM1

Yue Liu1, Jinwei Sun1, Yuhua Shi1

  • 1The Yancheng School of Clinical Medicine of Nanjing Medical University, Yancheng Third People's Hospital, Yancheng, Jiangsu, China.

Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research
|March 18, 2026
PubMed
Abstract

Insights

Family with sequence similarity 107 member A (FAM107A) inhibits colorectal cancer (CRC) cell growth and promotes apoptosis. The DNMT1/FAM107A/FOXM1 axis is identified as a key regulator in CRC development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Family with sequence similarity 107 member A (FAM107A) is known to suppress cancer progression.
  • The role of FAM107A in colorectal cancer (CRC) has not been previously investigated.

Purpose of the Study:

  • To explore the association between FAM107A and colorectal cancer (CRC).
  • To investigate the regulatory mechanism of FAM107A in CRC progression.

Main Methods:

  • Cellular aggressive behaviors were assessed using CCK-8, colony formation, EdU, and flow cytometry assays.
  • FAM107A promoter methylation and the interaction between DNMT1 and FAM107A were analyzed using bioinformatics, RT-qPCR, Western blot, and ChIP assays.

Main Results:

  • FAM107A overexpression significantly inhibited CRC cell proliferation, induced apoptosis, and caused cell cycle arrest.
  • DNMT1 was found to directly bind to the FAM107A promoter, and DNMT1 silencing enhanced FAM107A expression.
  • FOXM1 overexpression promoted CRC cell proliferation and tumor growth, which was reversed by FAM107A upregulation.

Conclusions:

  • The DNMT1/FAM107A/FOXM1 axis plays a crucial role in the occurrence and development of colorectal cancer (CRC).
  • FAM107A acts as a tumor suppressor in CRC, potentially through its interaction with DNMT1 and FOXM1.
Keywords:
Colorectal cancerFAM107AFOXM1methylationtumorigenesis

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