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Identification and Validation of Key Purine Metabolism-Related Genes in Ulcerative Colitis Using Bioinformatics and

Su Zhang¹, Yifang Zhang², Dongwei Du²

¹Department of Rheumatology, the Nanping First Affiliated Hospital of Fujian Medical University, Nanping, People's Republic of China.

Journal of Inflammation Research

|March 18, 2026

Summary

This summary is machine-generated.

Researchers identified PDE4B as a key biomarker for ulcerative colitis (UC) linked to purine metabolism. Inhibiting PDE4B expression may offer a new therapeutic strategy for UC by promoting gut barrier repair and reducing inflammation.

Keywords:

PDE4B bioinformatics immune infiltration machine learning purine metabolism ulcerative colitis

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Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

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Published on: November 30, 2016

Area of Science:

Gastroenterology
Immunology
Molecular Biology

Background:

Ulcerative colitis (UC) is a prevalent inflammatory bowel disease with complex pathogenesis, complicating diagnosis and treatment.
The precise role of purine metabolism in UC remains incompletely understood, despite its known association with various diseases.

Purpose of the Study:

To identify reliable biomarkers associated with purine metabolism in ulcerative colitis.
To elucidate the potential therapeutic targets within purine metabolism pathways for UC management.

Main Methods:

Utilized Gene Expression Omnibus (GEO) datasets to identify differentially expressed genes (DEGs) in UC.
Applied Weighted Gene Co-expression Network Analysis (WGCNA) to pinpoint key UC-associated gene modules.
Employed machine learning algorithms to screen and validate differentially expressed purine metabolism-related genes (UCDE-PMRGs), identifying PDE4B as a critical factor.

Main Results:

Identified 2133 DEGs and 9 UCDE-PMRGs, with PDE4B emerging as a key gene.
PDE4B expression was significantly correlated with immune cell infiltration and disease activity in UC patients.
Inhibition of PDE4B expression demonstrated therapeutic potential by enhancing intestinal epithelial barrier repair and alleviating UC symptoms in preclinical models.

Conclusions:

PDE4B serves as a significant biomarker for ulcerative colitis related to purine metabolism.
Targeting PDE4B presents a promising novel therapeutic avenue for managing UC, potentially by modulating purine metabolism and improving gut barrier function.