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SlMYB17 Antagonises the SlCBF Pathway to Negatively Regulate Tomato Chilling Tolerance
Ziyan Liu1,2, Zaidong Ge1, Si Jing1
1College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
Plant Biotechnology Journal
|March 18, 2026
Summary
We identified SlMYB17 as a key factor that reduces tomato chilling tolerance. Tomato mutants lacking SlMYB17 show improved cold resistance, offering potential for developing hardier tomato varieties.
Area of Science:
- Plant Biology
- Molecular Genetics
- Environmental Stress Physiology
Background:
- Low temperatures pose a significant challenge to cultivating tropical plants like tomatoes.
- Understanding the genetic mechanisms underlying plant chilling tolerance is crucial for crop improvement.
Purpose of the Study:
- To identify and characterize the role of the transcription factor SlMYB17 in regulating tomato chilling tolerance.
- To elucidate the molecular mechanisms by which SlMYB17 affects cold response pathways.
Main Methods:
- ChIP-seq analysis to identify SlMYB17 target genes.
- Protein-protein interaction assays and Dual-LUC assays to study molecular interactions.
- Virus-induced gene silencing (VIGS) to assess gene function in vivo.
Main Results:
- SlMYB17 acts as a negative regulator of tomato chilling tolerance; its overexpression decreases tolerance, while mutants show enhanced tolerance.
- SlMYB17 directly represses the expression of cold-regulated (COR) genes, including SlCOR27b and WCOR413.
- SlMYB17 interacts with SlCBF1, SlCBF2, and SlCBF3 to inhibit their activation of COR genes, functioning at both transcriptional and protein activity levels.
Conclusions:
- SlMYB17 antagonizes the SlCBF pathway, suppressing tomato chilling tolerance.
- SlMYB17 mutants are valuable genetic resources for breeding chilling-tolerant tomato varieties.
- The study provides insights into the role of MYB transcription factors in plant stress responses and development.
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