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Related Concept Videos

Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems01:22

Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems

315
Bioavailability is a critical pharmacological concept that measures the extent and rate at which an active drug ingredient or therapeutic moiety enters the systemic circulation, remaining unchanged. It's a pivotal factor in determining a drug's efficacy and safety.The Biopharmaceutics Classification System (BCS) plays an essential role in drug development by categorizing drugs into four classes based on their solubility and permeability. This classification aids in understanding drug absorption...
315

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Related Experiment Video

Updated: Mar 19, 2026

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples
09:51

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

Published on: September 19, 2025

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Bioassay-Based and Parameter-Optimized Bioaccessibility Assay to Predict Chromium Oral Bioavailability from

Wenjie Dong1, Can Liu1, Guobing Lin1

  • 1Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, and State Key Laboratory of Soil Pollution Control and Safety, Zhejiang University, Hangzhou 310058, Zhejiang, China.

Environmental Science & Technology
|March 18, 2026
PubMed
Summary
This summary is machine-generated.

A new mouse urine bioassay helps predict toxic chromium bioavailability in soil. Optimized in vitro methods improve accuracy for assessing human health risks from contaminated soil ingestion.

Keywords:
Cr relative bioavailabilityCr speciationSBRC, PBET and IVGhealth risk assessmentmouse urine bioassayoral exposureresponse surface methodology

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Area of Science:

  • Environmental Science
  • Toxicology
  • Biochemistry

Background:

  • Oral exposure to toxic chromium in contaminated soil poses significant human health risks.
  • Assessing chromium bioavailability is crucial for risk evaluation and remediation strategies.

Purpose of the Study:

  • To develop and validate an in vitro bioaccessibility assay for predicting chromium relative bioavailability (Cr-RBA) in soils.
  • To optimize assay parameters for improved prediction accuracy of in vivo Cr-RBA.

Main Methods:

  • A mouse urine bioassay was used to determine Cr-RBA in soils.
  • Three in vitro bioaccessibility assays (SBRC, PBET, IVG) were evaluated against in vivo data.
  • Response surface methodology with Box-Behnken Design was employed to optimize the SBRC-GP assay parameters.

Main Results:

  • Initial in vitro assays showed variable Cr bioaccessibility and moderate correlations with in vivo Cr-RBA.
  • The optimized SBRC-GP assay (pH 1.2, 1:150 S/L ratio, 2h) significantly increased Cr bioaccessibility (2.3-4.2 fold).
  • The modified assay demonstrated improved prediction of soil Cr-RBA (R² = 0.66, slope = 1.0).

Conclusions:

  • An optimized SBRC-GP in vitro bioaccessibility assay effectively predicts chromium bioavailability in soils.
  • This optimized assay aids in assessing health risks from soil ingestion and refining remediation goals for chromium-contaminated sites.