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Protocol for preparing tyramine functionalized hyaluronic acid via aqueous DMTMM-mediated conjugation
Gregor Miklosic1, Daphne van der Heide1, Claudia Loebel2
1AO Research Institute Davos, 7270 Davos, Switzerland; Institute for Biomechanics, ETH Zürich, 8092 Zürich, Switzerland.
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Hyaluronic acid is a ubiquitous glycosaminoglycan with numerous biological and structural functions in the body. Here, we present a protocol for the practical and efficient functionalization of HA with tyramine. We describe the reaction conditions for its DMTMM-mediated coupling, the subsequent purification and drying steps, and product characterization through 1H NMR and UV spectrophotometry. This protocol produces materials which can form hydrogels with tunable viscoelastic properties, for a range of biomedical applications, such as biofabrication and tissue engineering. For complete details on the use and execution of this protocol, please refer to Miklosic et al.1.
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