Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond classification metrics: a psychometric-aware benchmark for data augmentation in imbalanced student mental health surveys.

Frontiers in digital health·2026
Same author

Spatially resolved profiling of extracellular vesicles in tissues with Spatial-EV-seq.

Nature biotechnology·2026
Same author

School and family based myopia education associations with myopia prevalence in Hefei high school students in the post-COVID-19 period.

Scientific reports·2026
Same author

Cyclic transformation of stable/metastable nucleic acid structures enables dynamic monitoring of ATP in living cells.

Chemical science·2026
Same author

Dual-Spatially Confined Assembly of DNA Nanowall Stiffens Tumor Cells to Enhance Adoptive T-Cell Immunotherapy.

Journal of the American Chemical Society·2026
Same author

Tracing Tumor-Derived Extracellular Vesicle Matrix Metalloproteinase 14 Using Dual-Target Orthogonal Barcoding-Based Microscale Thermophoretic Assays.

ACS nano·2026
Same journal

Deep Learning Network-Tailored Microenvironment Matching of 4D Bioprinting Bioactive Scaffolds for Bone Regeneration.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Autonomous High-Throughput Characterization of Liquid-Liquid Phase Behavior.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Laser Preset of MnO<sub>x</sub> Layer on High-Entropy Alloy Surface for Ampere-Level Ultra-Stable OER Performance.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

PDGFRα<sup>+</sup>/Integrin α2<sup>+</sup> Fibroblasts Orchestrate Tumor Budding in Oral Squamous Cell Carcinoma via Mechano-Metabolic Symbiosis: E-Cadherin/Integrin α2β1 Adhesion and Mitochondrial Transfer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Synergistic Ni Single Atoms/Nanoparticles on CeO<sub>2</sub> for High-Performance and Durable SOFC Hydrogen Electrodes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

A Review of Failure Modes and Safety Strategies of Lithium-Ion Batteries from Materials to Systems.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
See all related articles

Related Experiment Video

Updated: Mar 20, 2026

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
10:23

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

Published on: November 4, 2022

3.6K

RENAL-CHIP: Rejection Evaluation via Non-Invasive Analysis of Circulating Podocytes With Herringbone-Chip Isolation

Juan Song1, Yilong Liu2, Zeyuan Zheng1

  • 1Discipline of Intelligent Instrument and Equipment, College of Chemistry and Chemical Engineering, Department of Urology, Xiang'an Hospital of Xiamen University, School of Medicine, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|March 18, 2026
PubMed
Summary
This summary is machine-generated.

Donor-derived circulating podocytes (CPCs) in blood can now non-invasively detect kidney transplant rejection. Enumerating CPCs offers a precise, early warning for renal allograft recipients.

Keywords:
circulating podocytesimmune rejectionmicrofluidic interfacenon‐invasive diagnosisrenal transplant

More Related Videos

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
05:58

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

1.8K
Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research
08:48

Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research

Published on: May 24, 2024

2.8K

Related Experiment Videos

Last Updated: Mar 20, 2026

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
10:23

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

Published on: November 4, 2022

3.6K
Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
05:58

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

1.8K
Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research
08:48

Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research

Published on: May 24, 2024

2.8K

Area of Science:

  • Nephrology
  • Transplantation Immunology
  • Biomarker Discovery

Background:

  • Long-term renal allograft survival is compromised by immune rejection.
  • Current diagnostic methods for rejection lack non-invasive precision.
  • Need for reliable, early detection of kidney transplant rejection.

Purpose of the Study:

  • To identify donor-derived circulating podocytes (CPCs) as a blood-based biomarker for acute renal allograft rejection.
  • To develop a minimally invasive method for detecting kidney transplant rejection.

Main Methods:

  • Development of a magnetically reversible microfluidic chip for efficient isolation of CPCs using EpCAM-functionalized magnetic beads.
  • Enumeration of CPCs in peripheral blood samples from kidney transplant recipients.
  • Confirmation of donor origin using fluorescence in situ hybridization (FISH) in sex-mismatched transplants.
  • Single-cell RNA sequencing (scRNA-seq) to analyze gene expression in isolated CPCs.

Main Results:

  • High efficiency (84.4%) and viability (96.0%) in isolating CPCs from 1 mL of blood.
  • A threshold of ≥35 CPCs/mL accurately identified biopsy-confirmed rejection (AUC = 1.0) in 65 participants.
  • scRNA-seq revealed upregulated podocyte injury and immune-related genes (NF-κB, TNF, NOD-like receptors) in CPCs from rejection cases.

Conclusions:

  • Donor-derived circulating podocytes (CPCs) serve as a sensitive and specific non-invasive biomarker for acute renal allograft rejection.
  • CPC enumeration provides a mechanistically informed, minimally invasive warning of kidney transplant rejection.
  • This approach can improve long-term renal allograft survival through early detection and intervention.