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Tracing Tumor-Derived Extracellular Vesicle Matrix Metalloproteinase 14 Using Dual-Target Orthogonal Barcoding-Based

Aipeng Chen1, Ruoke Wang1, Yan Zou1

  • 1School of Pharmacy, Fudan University, Shanghai 200438, P. R. China.

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Summary
This summary is machine-generated.

A new assay detects matrix metalloproteinase 14 (MMP14) on tumor extracellular vesicles (EVs) in breast cancer. This ultrafast, one-step dual-target orthogonal barcoding-based microscale thermophoretic extracellular vesicle (DOT-EV) assay aids in early diagnosis and metastasis prediction.

Keywords:
cancer metastasisdual-target orthogonal labelingextracellular vesiclesliquid biopsymicroscale thermophoresis assay

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Area of Science:

  • Biomedical Engineering
  • Molecular Diagnostics
  • Cancer Research

Background:

  • Matrix metalloproteinase 14 (MMP14) on extracellular vesicles (EVs) is crucial for breast cancer invasiveness.
  • Detecting MMP14 in complex blood samples is challenging due to abundant normal EVs, limiting sensitivity and specificity.

Purpose of the Study:

  • To develop an ultrafast, highly specific assay for quantifying MMP14 on tumor-derived EVs (T-EVs).
  • To enable precise monitoring of breast cancer progression and metastasis prediction through T-EV MMP14 profiling.

Main Methods:

  • Developed an ultrafast one-step dual-target orthogonal barcoding-based microscale thermophoretic extracellular vesicle (DOT-EV) assay.
  • Utilized orthogonal barcoding with aptamer probes targeting MMP14 and EpCAM for specific T-EV recognition.
  • Employed microscale thermophoresis for rapid DNA assembly and detection within 20 seconds using 10 μL plasma.

Main Results:

  • The DOT-EV assay accurately detected T-EV MMP14 levels in metastatic mouse models and human breast cancer patient samples.
  • Demonstrated ability to monitor tumor development stages and predict metastatic potential.
  • Successfully differentiated triple-negative breast cancer from other subtypes.

Conclusions:

  • The DOT-EV assay provides a pioneering tool for noninvasive cancer diagnosis and progression monitoring.
  • Offers precise metastasis prediction, showing promise for advancing precision medicine in oncology.
  • Enables rapid, sensitive, and specific detection of T-EV MMP14 for improved clinical outcomes.