Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Microbiota Modulation by Antibiotics01:21

Microbiota Modulation by Antibiotics

Antibiotics have revolutionized modern medicine by saving countless lives from bacterial infections. However, their widespread use has inadvertently harmed the delicate balance of the human gut microbiota. The gut microbiota, a complex community of bacteria, archaea, viruses, and fungi, plays a vital role in regulating metabolism, immune responses, and maintaining intestinal health. Antibiotics, especially broad-spectrum types, disrupt this ecosystem by eradicating both harmful and beneficial...
Methods to Assess Microbial Communities01:19

Methods to Assess Microbial Communities

Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...
Antibiotic Selection00:57

Antibiotic Selection

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatially resolved profiling of extracellular vesicles in tissues with Spatial-EV-seq.

Nature biotechnology·2026
Same author

Cyclic transformation of stable/metastable nucleic acid structures enables dynamic monitoring of ATP in living cells.

Chemical science·2026
Same author

Dual-Spatially Confined Assembly of DNA Nanowall Stiffens Tumor Cells to Enhance Adoptive T-Cell Immunotherapy.

Journal of the American Chemical Society·2026
Same author

Tracing Tumor-Derived Extracellular Vesicle Matrix Metalloproteinase 14 Using Dual-Target Orthogonal Barcoding-Based Microscale Thermophoretic Assays.

ACS nano·2026
Same author

Discussion of the practice of centralized multicenter integration of independent medical laboratory test item classification.

Laboratory medicine·2026
Same author

Low-Dose Aspirin Recommendations for Preeclampsia Risk Reduction: A Systematic Review Focused on Black Patients : Current Hypertension Reports.

Current hypertension reports·2026

Related Experiment Video

Updated: Jul 17, 2026

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

CommAST Enables Antibiotic Susceptibility Testing at the Gut Microbiota Community Level.

Chenling Ding1, Weiqi Wu2,3, Yanqi Zhang4,5

  • 1Department of Critical Care Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.

Analytical Chemistry
|July 15, 2026
PubMed
Summary

New CommAST technology measures antibiotic susceptibility in gut bacteria, even uncultured species. It reveals higher community-level resistance than previously known, improving understanding of antibiotic-induced gut dysbiosis.

More Related Videos

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
08:33

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Published on: November 17, 2018

High Throughput Co-culture Assays for the Investigation of Microbial Interactions
07:00

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Published on: October 15, 2019

Related Experiment Videos

Last Updated: Jul 17, 2026

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
08:33

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Published on: November 17, 2018

High Throughput Co-culture Assays for the Investigation of Microbial Interactions
07:00

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Published on: October 15, 2019

Area of Science:

  • Microbiology
  • Systems Biology
  • Drug Discovery

Background:

  • Gut microbiota dysbiosis is a common consequence of oral antibiotic use.
  • Traditional antimicrobial susceptibility testing (AST) methods fail for unculturable gut bacteria and complex microbial communities.
  • Assessing antibiotic responses within the gut ecosystem requires novel approaches.

Purpose of the Study:

  • To develop and validate a novel platform, CommAST (Community-level Omni-species Metabolic-activity Antibiotic Susceptibility Testing), for profiling antibiotic susceptibility in complex microbial communities.
  • To determine the minimum inhibitory concentrations (MICs) for multiple bacterial species simultaneously within a microbial ecosystem.
  • To investigate discrepancies between strain-level and community-level antibiotic resistance.

Main Methods:

  • CommAST utilizes serial antibiotic concentrations and fluorescent D-amino acid labeling to assess metabolic activity in individual cells.
  • Flow cytometry quantifies cellular metabolic status, while fluorescence in situ hybridization (FISH) identifies species identity.
  • The platform was applied to human fecal microbiota samples to profile drug-bacteria interactions.

Main Results:

  • CommAST successfully profiled MICs for 105 drug-bacteria combinations across 27 species in human fecal microbiota, including previously uncultured taxa.
  • Significant discrepancies were observed, with community-level MICs often exceeding strain-level MICs.
  • Transcriptomic analysis indicated synergistic mechanisms like biofilm protection and metabolic cost-sharing contribute to community tolerance.

Conclusions:

  • CommAST is an innovative platform for community-level AST, enabling susceptibility profiling of numerous species, including unculturable ones.
  • The study highlights that gut bacteria exhibit higher tolerance to antibiotics at the community level than individually.
  • Understanding these community-level resistance mechanisms is crucial for addressing antibiotic-induced gut dysbiosis.